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Analysis, gene cloning and expression of two α-amylases from Bacillus cereus

✍ Scribed by Lin-lin, Zhang; Qi, Wang; Rong-xi, Li; Yong-jun, Wang; Ru-hong, Mei

Book ID
115456010
Publisher
Cambridge University Press
Year
2009
Tongue
English
Weight
642 KB
Volume
6
Category
Article
ISSN
1479-2362

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✍ Tsukagoshi, N. ;Ihara, H. ;Yamagata, H. ;Udaka, S. 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 584 KB

A 6.4 Kb HindIII fragment of Bacillus stearothermophilus DY-5 DNA cloned in Escherichia coli using pBR322 as a vector was shown to direct the synthesis of a thermophilic alpha-amylase. In attempts to reduce the size of the insert, the alpha-amylase gene was shown to be contained in a 3.1 Kb HindIII