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Investigation of Newly Synthesized Endothelin Peptides by High-performance Liquid Chromatography Coupled to Electrospray Mass Spectrometry

✍ Scribed by Luca Rovatti; Ornella Curcuruto; Mahmoud Hamdan; Ersilia Cassano; Claudia Galoppini; Paolo Rovero

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 367 KB
Volume: 10
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(199609)10:12<1504::aid-rcm640>3.0.co;2-5

No coin nor oath required. For personal study only.

✦ Synopsis

A number of newly synthesized endothelin peptides have been examined by high performance liquid chromatographylelectrospray mass spectrometry (HPLC/ES-MS). The presented data allowed reliable identification of both the target peptides and a number of associated side products including the target peptide in methylated form and peptide sequences with relative molecular masses lower than those desired. Characteristic fragments in the ES m a s spectra permitted differentiation between the methylation sites involving the earboxylic functions of the C-terminus and the side-chain of the aspartic acid.

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