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Investigation of Newly Synthesized Peptides by Capillary Zone Electrophoresis/Electrospray Mass Spectrometry

✍ Scribed by Mahmoud Hamdan; Ornella Curcuruto; Stefania Vigano'; Paolo Rovero

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 320 KB
Volume: 10
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(19960715)10:9<1128::aid-rcm589>3.0.co;2-b

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✦ Synopsis

A series of newly synthesized peptides (M,= 1600-2250 Da), corresponding to portions of the extracellular domain of human granulocyte-macrophage colony stimulating factor receptor a subunit have been examined by capillary zone electrophoresis/electrospray-mass spectrometry (CZE/ES-MS). The separation efficiency of CZE combined with the specificity of mass spectrometry allowed rapid and reliable identification of the target peptides and a number of associated side products. In solid-phase synthesis of peptides and small proteins such side products are inevitable, therefore the use of CZE and/or high-performance liquid chromatography combined with mass spectrometry is gaining a central role in such synthesis procedures.

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