Interaction of thiamine with reducing sugars
โ Scribed by L. Doyon; T.G. Smyrl
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 312 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0308-8146
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๐ SIMILAR VOLUMES
The interaction between isoniazid and reducing sugars is acid-catalyzed and reversible. Kinetic studies of hydrazone formation from isoniazid and glucose, lactose, maltose, and galactose have been carried out in simulated gastric juice at 37OC. The forward reaction was found to follow second-order k
Many polysaccharide-degrading enzymes are commonly assayed by quantifying the amount of reducing sugars released during the assay. Typically the reducing sugars are quantified by one of a number of colorimetric procedures (1-4). Colorimetric assays based on
The photometric assay of reducing sugars with 2,2'-bicinchoninate was improved and its conditions were optimized. Optical density is linear between 1 and 25 nmol sugar/sample, and the assay is not affected by borate, phosphate, or other buffer anions. The molar extinction coefficients produced by th