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Assay of reducing sugars in the nanomole range with 2,2′-bicinchoninate

✍ Scribed by Sabine Waffenschmidt; Lothar Jaenicke

Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
285 KB
Volume
165
Category
Article
ISSN
0003-2697

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✦ Synopsis

The photometric assay of reducing sugars with 2,2'-bicinchoninate was improved and its conditions were optimized. Optical density is linear between 1 and 25 nmol sugar/sample, and the assay is not affected by borate, phosphate, or other buffer anions. The molar extinction coefficients produced by the standard procedure with the 12 most common monosaccharides occurring in carbohydrates and conjugated glycocompounds are listed.

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