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Colorimetric estimation of ethanolamine with p-benzoquinone in the nanomole range

✍ Scribed by Annick Le Dur; Richard Chaby; Ladislas Szabó

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
462 KB
Volume
88
Category
Article
ISSN
0003-2697

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✦ Synopsis

Ethanolamine reacts with p-benzoquinone in alkaline medium to give a product of undetermined structure, the absorption of which at 348 nm can be used for the estimation of the base. Conditions are described in which the interference of 0-phosphorylethanolamine, glucosamine, and neutral sugars is eliminated. The method was used to estimate the ethanolamine content of phospholipids alone and admixed to interfering substances. It is unreliable in the presence of amino acids or proteins.

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