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Determination of Reducing Sugars with 3-Methyl-2-benzothiazolinonehydrazone

โœ Scribed by Gordon E. Anthon; Diane M. Barrett

Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
39 KB
Volume
305
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

Many polysaccharide-degrading enzymes are commonly assayed by quantifying the amount of reducing sugars released during the assay. Typically the reducing sugars are quantified by one of a number of colorimetric procedures (1-4). Colorimetric assays based on

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A manual method for reducing sugar deter
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โœ R.F. McFeeters ๐Ÿ“‚ Article ๐Ÿ“… 1980 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 385 KB

A method was developed to analyze reducing sugars manually by use of a 2,2'bicinchoninate reagent. Potassium phosphate was used to buffer the reagent. Ethylene glycol increased the sensitivity of the assay for several sugars. Sugar determinations can be performed in the presence of borate ion. In co

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The photometric assay of reducing sugars with 2,2'-bicinchoninate was improved and its conditions were optimized. Optical density is linear between 1 and 25 nmol sugar/sample, and the assay is not affected by borate, phosphate, or other buffer anions. The molar extinction coefficients produced by th