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A manual method for reducing sugar determinations with 2,2′-bicinchoninate reagent

✍ Scribed by R.F. McFeeters

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
385 KB
Volume
103
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method was developed to analyze reducing sugars manually by use of a 2,2'bicinchoninate reagent. Potassium phosphate was used to buffer the reagent. Ethylene glycol increased the sensitivity of the assay for several sugars. Sugar determinations can be performed in the presence of borate ion. In comparative assays of a number of monosaccharides and disaccharides, the bicinchoninate reagent was about as sensitive as the Nelson reagent but more convenient to use. The effects of ethylene glycol concentration, reagent pH, heating time, and borate ion on color development were evaluated.

' Mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by the U. S. Department of Agriculture or North Carolina State University, nor does it imply approval to the exclusion of other products that may be suitable.

* Abbreviations used: solution A,, prepared by dissolving 160.9 g K,HPO,, 10.4 g KH,PO+ and 870 mg 2,2'-bicinchoninate in water and diluting to 1 liter, pH 8.5; solution A,, same as A,, but containing 333 ml/ liter ethylene glycol; solution B, a solution of 25 g

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