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A manual method for the spectrophotometric determination of reducing carbohydrates with 2-cyanoacetamide

โœ Scribed by Susumu Honda; Yoshiko Nishimura; Masaye Takahashi; Hirokazu Chiba; Kazuaki Kakehi

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
389 KB
Volume
119
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A sample solution (1 ml) of D-ghCOSe, a 1% aqueous solution ( 1 ml) of 2-cyanoacetamide, and 0.1 M borate buffer (pH 9.0, 2 ml) are mixed and heated for 10 min in a boiling-water bath. Measurement of the absorbance of the reaction mixture at 276 nm allows the rapid and simple determination of 5 to 750 nmol of D-ghicose with errors and coefficients of variation less than 0.4 and 2.6'5, respectively. Other reducing carbohydrates give similar results. Relative molar absorbances for various carbohydrates are compared and discussed in relation to their structures. Interference with some biochemical compounds is also investigated.

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