A sample solution (1 ml) of D-ghCOSe, a 1% aqueous solution ( 1 ml) of 2-cyanoacetamide, and 0.1 M borate buffer (pH 9.0, 2 ml) are mixed and heated for 10 min in a boiling-water bath. Measurement of the absorbance of the reaction mixture at 276 nm allows the rapid and simple determination of 5 to 7
Reaction of glutaraldehyde with NH2 compounds: A spectrophotometric method for the determination of glutaraldehyde concentration
✍ Scribed by János Hajdu; Peter Friedrich
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 396 KB
- Volume
- 65
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Glut~aIde~yde reacts with an excess of hydroxy~am~ne, hydrazine or methytamine through an intermediate that absorbs more strongly at 240 nm than do the end products. On the basis of the characteristic absorbance change during the reaction with hydroxylamine a spectrophotometric difference method was developed for the determination of glutaraldehyde concentration which is not disturbed by the presence of other materials absorbing in the ultraviolet.
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