A fluorometric method for the specific determination of serum arginine with 2,3-naphthalenedicarbaldehyde

✍ Scribed by Toshiaki Miura; Megumi Kashiwamura; Michiya Kimura

• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 474 KB
• Volume: 139
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(84)90030-7

✦ Synopsis

A specific and simple fluorometric method for the determination of serum arginine was developed which consists of (1) deproteinization of serum sample with 10% perchloric acid, (2) blocking thiol compounds with N-ethylmaleimide, (3) fluorescence reaction with 2,3-naphthalenedicarbaldehyde in 0.2 M borate buffer, pH 9.3, in the presence of beta-cyclodextrin (25 min at 20 degrees C, excitation wavelength: 462 nm, emission wavelength: 520 nm). Since the fluorescence reaction is highly specific for arginine, the method requires no other separation step. Linearity, recovery, and reproducibility of the method were found to be satisfactory.