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InsP3 mediates acetylcholine-induced cytosolic Ca2+ release in mouse C2C12 myotubes

✍ Scribed by Aldo Giovannelli; Cristina Limatola; Elisabetta Mattei; Francesca Grassi; Davide Ragozzino; Fabrizio Eusebi

Book ID
113401619
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
70 KB
Volume
88
Category
Article
ISSN
0928-4257

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In cultured mouse C2C12 myotubes, digital Ca 2+ imaging fluorescence microscopy using the acetoxymethyl ester of Fura-2, Fura-2-AM, showed that, in the absence of extracellular Ca 2+, acetylcholine (ACh) and nicotine, but not muscarine, raised the intracellular concentration of Ca 2 + ([Ca 2 +]i) by