Agrin induces association of Chrna1 mRNA and nicotinic acetylcholine receptor in C2C12 myotubes
β Scribed by Yung-Fu Chang; Hui-Ju Chou; Ying-Cheng Yen; Hsueh-Wei Chang; Yi-Ren Hong; Hurng-Wern Huang; Chao-Neng Tseng
- Book ID
- 118442947
- Publisher
- Elsevier Science
- Year
- 2012
- Tongue
- English
- Weight
- 688 KB
- Volume
- 586
- Category
- Article
- ISSN
- 0014-5793
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## Abstract Protein kinase C (PKC) activity has been implicated in nicotinic acetylcholine receptor (nAChR) cluster disruption but the specific PKC isoforms involved have not been identified. We first tested whether phorbol esters, which activate PKCs, regulate agrinβinduced nAChR clustering in C~2
In cultured mouse C2C12 myotubes, digital Ca 2+ imaging fluorescence microscopy using the acetoxymethyl ester of Fura-2, Fura-2-AM, showed that, in the absence of extracellular Ca 2+, acetylcholine (ACh) and nicotine, but not muscarine, raised the intracellular concentration of Ca 2 + ([Ca 2 +]i) by