Caffeine and nicotine decrease acetylcholine receptor clustering in C2C12 myotube culture
β Scribed by Kaia Kordosky-Herrera; Wade A. Grow
- Book ID
- 106139565
- Publisher
- Springer-Verlag
- Year
- 2008
- Tongue
- English
- Weight
- 319 KB
- Volume
- 335
- Category
- Article
- ISSN
- 0302-766X
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In cultured mouse C2C12 myotubes, digital Ca 2+ imaging fluorescence microscopy using the acetoxymethyl ester of Fura-2, Fura-2-AM, showed that, in the absence of extracellular Ca 2+, acetylcholine (ACh) and nicotine, but not muscarine, raised the intracellular concentration of Ca 2 + ([Ca 2 +]i) by
## Abstract Protein kinase C (PKC) activity has been implicated in nicotinic acetylcholine receptor (nAChR) cluster disruption but the specific PKC isoforms involved have not been identified. We first tested whether phorbol esters, which activate PKCs, regulate agrinβinduced nAChR clustering in C~2