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Inhibitory effects of legume seed extracts on fish proteinases

✍ Scribed by Benjakul, Soottawat; Karoon, Somkid; Suwanno, Angkana

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 427 KB
Volume: 79
Category: Article
ISSN: 0022-5142
DOI: 10.1002/(sici)1097-0010(199910)79:13<1875::aid-jsfa447>3.0.co;2-u

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✦ Synopsis

Proteinase inhibitor extracts were prepared from various legume seeds. Black cowpea and soybean seeds showed the highest inhibitory activity against viscera proteinases (p `0.05). Optimal extraction was attained by shaking the defatted legume seed powder in distilled water (3 ml g À1 ) at ambient temperature for 1 h. Both extracts showed high thermal stability, but that from soybean was slightly less stable. The inhibitory activity of both extracts was retained over a broad pH range. The proteinase inhibitor extracts also reduced modori-inducing proteinase (MIP) activity. The sarcoplasmic MIP activities were more effectively inhibited than the myo®brillar-associated MIP activities.

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