Inhibitory action of spray dried blood plasma and whole egg powder on lectins in extracts of several legume seeds: a qualitative approach
✍ Scribed by Van Nevel, Christian; De Rycke, Herman; Beeckmans, Sonia; De Wilde, Roland; Van Driessche, Edilbert
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 253 KB
- Volume
- 77
- Category
- Article
- ISSN
- 0022-5142
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✦ Synopsis
Samples of seeds from eight legume species and T riticum vulgaris grains were extracted with bu †er and lectin activity in the extracts was determined in haemagglutination experiments using normal or Pronase-treated rabbit erythrocytes. The e †ect of the addition of spray dried porcine and bovine plasma powder, whole egg powder, galactosides, whey powder and speciÐc inhibitors (eg mannose, galactose, N-acetyl galactosamine, fetuin) on haemagglutination activity (HA) was determined. Plasma powders were potent inhibitors of HA in extracts of Pisum sativum, V icia faba, V icia sativa, L ens culinaris and Phaseolus vulgaris. HA in extracts of L upinus sp and Phaseolus vulgaris was efficiently decreased by whole egg powder, while the lectin of Glycine max could only be inhibited by addition of galactosides, whole and defatted milk powder and whey powder. Inhibitors (plasma and whole egg powder and fetuin) were subjected to SDS-PAGE and Western blotting and blots were incubated with biotinylated lectins, except for L upinus lectin. Results of the HA experiments were conÐrmed : lectins which were not inÑuenced by inhibitory compounds in HA experiments also showed no binding with proteins of the blotted inhibitor. There were strong indications that lectins were not bound to the albumin fraction of the plasma powders. Results are discussed in view of future in vivo experiments.
1998 SCI.