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Inhibitory Effect of Sucrose on the Plasma Membrane H+Pump Activity and Possible Involvement of the C-terminal Region in the Sucrose Effect

โœ Scribed by Minobu Kasai; Kyoko Sato; Tsuyoshi Ono; Takako Ono; Kanji Kasai; Sachio Tsuruta; Miho Kikuchi; Junichi Yamazaki; Nobuyuki Kanazawa; Shinichi Sawada


Publisher
Springer
Year
2000
Tongue
English
Weight
566 KB
Volume
113
Category
Article
ISSN
0918-9440

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The plasma membrane ATPase ofKloeckera a
โœ H. Alexandre; C. Charpentier ๐Ÿ“‚ Article ๐Ÿ“… 1994 ๐Ÿ› Springer ๐ŸŒ English โš– 515 KB

Partially (6-fold) purified plasma membrane ATPase from an ethanol-sensitive yeast, Kloeckera apiculata, had an optimum pH of 6.0, an optimum temperature of 35ยฐC, a K m of 3.6 mM ATP and a V max of 11 ฮผmol Pi/min.mg protein. SDS-PAGE of the semi-purified plasma membrane showed a major band of 106 kD