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Inhibition of DNA-induced transformation by concanavalin A in Bacillus subtilis

โœ Scribed by Cho-yam Chu; Kuo-chun Chen


Book ID
118313314
Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
385 KB
Volume
91
Category
Article
ISSN
0006-291X

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High frequency transformation of Bacillu
โœ Chang, Shing ;Cohen, Stanley N. ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Springer ๐ŸŒ English โš– 449 KB

A highly efficient method for transformation of Bacillus subtilis by plasmid DNA is reported. The procedure, which involves polyethylene glycol-induced DNA uptake by protoplasts and subsequent regeneration of the bacterial cell wall, yields up to 80% transformants with an efficiency of 4 x 10(7) tra