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Influence of the culture oxygenation on the release of LDH by hybridoma cells

✍ Scribed by V. Geaugey; F. Pascal; J. M. Engasser; A. Marc

Publisher
Springer-Verlag
Year
1990
Tongue
English
Weight
341 KB
Volume
4
Category
Article
ISSN
0951-208X

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✦ Synopsis

The viability of hybridoma cells grown in reactors is investigated with two methods: the Trypan Blue staining of dead cells and the release of the cytoplasmic lactate dehydrogenase (LDH). Linear correlations are found between the number of counted dead cells and the measured LDH activity in the supematant both when cells are grown with and without oxygen limitations. However the release of LDH is significantly higher for the oxygen limited culture ( 620 IU LDH / lo9 cells ) than for the non-oxygen limited culture ( 270 IU LDH / lo9 cells ).

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The comparative effects of Pluronic on cell growth of hybridomas were studied. The addition of 0.05% Pluronic decreased the steady-state cell number in continuous culture by about 12% compared to a non-supplemented culture. In short-term experiments, results demonstrated a gradual decrease in cell n