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The effect of Pluronic F-68 on hybridoma cells in continuous culture

✍ Scribed by Mohamed Al-Rubeai; A. N. Emery; S. Chalder

Publisher
Springer
Year
1992
Tongue
English
Weight
212 KB
Volume
37
Category
Article
ISSN
1432-0614

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✦ Synopsis

The comparative effects of Pluronic on cell growth of hybridomas were studied. The addition of 0.05% Pluronic decreased the steady-state cell number in continuous culture by about 12% compared to a non-supplemented culture. In short-term experiments, results demonstrated a gradual decrease in cell number with increasing concentration of Pluronic. Such growth inhibition was found to be a result of lowering the rate of DNA synthesis.

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## Abstract The surfactant Pluronic F‐68 (PF‐68) is widely used in large‐scale mammalian cell culture to protect cells from shear stress that arises from agitation and gas sparging. Several studies suggested that PF‐68 is incorporated into the cell plasma membrane and could enter the cells, but wit