𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Influence of pressure on solute retention in liquid chromatography

✍ Scribed by Victoria L. McGuffin; Christine E. Evans

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
612 KB
Volume
3
Category
Article
ISSN
1040-7685

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Although the mobile phase is generally considered to be incompressible in liquid chromatography, the local pressure appears to have a significant effect upon solute retention. In this investigation, laser fluorescence detection was utilized to measure solute retention directly along a reversed‐phase packed capillary column with a methanol mobile phase. For derivatized fatty acids, n‐C~10:0~ to n‐C~20:0~, the capacity factors increased systematically by +9.3% to + 24.4% under normal operating pressure conditions (1,500 to 5,000 psi). Whereas the magnitude of this increase in solute retention seems surprising, it appears to correlate well with the unified theoretical model developed by Martire.

πŸ“œ SIMILAR VOLUMES

Direct examination of separation process
Direct examination of separation processes in liquid chromatography: Effect of temperature and pressure on solute retention
✍ Victoria L. McGuffin; Christine E. Evans; Shu-Hui Chen πŸ“‚ Article πŸ“… 1993 πŸ› John Wiley and Sons 🌐 English βš– 680 KB

## Abstract In this study, retention processes in reversed‐phase liquid chromatography were examined __in situ__ along an optically transparent column by means of laser‐induced fluorescence. Using a homologous series of derivatized fatty acids as model solutes, the capacity factors were systematica

Pressure effects on relative retention i
Pressure effects on relative retention in capillary gas-liquid chromatography
✍ Victor G. Berezkin; Alexander A. Korolev; Irina V. Malyukova πŸ“‚ Article πŸ“… 1996 πŸ› John Wiley and Sons 🌐 English βš– 692 KB

The dependence of retention values (capacity factor, relative retention, Kovats' retention index) of some organic compounds (different chemical classes) on average column pressure has been experimentally studied for three carrier gases (hydrogen, nitrogen, and carbon dioxide). Linear equations for t

Influence of pressure on the retention o
Influence of pressure on the retention of sugars in hydrophilic interaction chromatography
✍ Uwe D. Neue; Chris J. Hudalla; Pamela C. Iraneta πŸ“‚ Article πŸ“… 2010 πŸ› John Wiley and Sons 🌐 English βš– 189 KB πŸ‘ 1 views

## Abstract Pressure can influence the retention of analytes in hydrophilic interaction chromatography as well as in RP chromatography. We demonstrate that the retention of sugars in hydrophilic interaction chromatography decreases with pressure, and interpret the observation as a gain in solvation

Solvent modulation: An accurately modele
Solvent modulation: An accurately modeled method for controlling solute retention in liquid chromatography
✍ Wahl, J. H. ;Enke, C. G. ;McGuffin, V. L. πŸ“‚ Article πŸ“… 1988 πŸ› John Wiley and Sons 🌐 English βš– 351 KB

An innovative solvent delivery method termed solvent modulation has been developed to control solute retention in liquid chromatography. Solvent modulation is the technique whereby individual solvent zones are introduced onto the chromatographic column in a known random or repeating sequence. Becaus