The solute retention mechanism of reversed-phase liquid chromatography is examined by using newly synthesized hydrophilic porous copolymer beads as a stationary phase. It is shown that when water is used as an eluent, the retention time for solutes such as alcohols, ketones and polyethylene glycols
Temperature effects on retention in reversed phase liquid chromatography
โ Scribed by Johnny V. Tran; Paal Molander; Tyge Greibrokk; Elsa Lundanes
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 213 KB
- Volume
- 24
- Category
- Article
- ISSN
- 1615-9306
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Reversed-phase packed capillary column liquid chromatography LC was investigated at column temperatures of 24ะC, 40ะC, 60ะC, and 80ะC. For a ลฝ . mixed mobile phase containing acetonitrile and water 72:28, vrv , the viscosity of the mobile phase decreased 2.2 times and the diffusion rate of the solut
While the choice of stationary phase, organic modifier, and gradient strength can have significant effects on biomolecule separations, mobile phase additives can also have a significant effect on the chromatographic selectivity, recovery, efficiency and resolution. Given the importance of stationary