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Influence of pressure on the retention of sugars in hydrophilic interaction chromatography

โœ Scribed by Uwe D. Neue; Chris J. Hudalla; Pamela C. Iraneta

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
189 KB
Volume
33
Category
Article
ISSN
1615-9306

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โœฆ Synopsis

Abstract

Pressure can influence the retention of analytes in hydrophilic interaction chromatography as well as in RP chromatography. We demonstrate that the retention of sugars in hydrophilic interaction chromatography decreases with pressure, and interpret the observation as a gain in solvation, or more specifically hydration, as the sugar molecules enter the waterโ€rich stationary phase.

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