Induction of the manganese-containing superoxide dismutase in Escherichia coli by nalidixic acid and by iron chelators
β Scribed by Hosni M. Hassan; Carmella S. Moody
- Book ID
- 109312730
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- English
- Weight
- 228 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0378-1097
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An Escherichia coli K-12 strain deleted for sodA and sodB (manganese and iron superoxide dismutases) was constructed and characterized by Southern blotting, enzyme assays, and physiological analyses. The sod deletion strain was used to clone the iron superoxide dismutase gene of Legionella pneumophi
## Abstract The SOS chromotest is a simple colorimetric genotoxicity assay that monitors DNA repair by measuring the induction of the gene __sfiA__ in __Escherichia coli__ Kβ12. __E. coli__ PQ300, a diagnostic SOS tester strain for the detection of oxidative genotoxins, carries a mutation in a key