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Roles of manganese and iron in the regulation of the biosynthesis of manganese-superoxide dismutase in Escherichia coli

โœ Scribed by Hosni M. Hassan; Laura W. Schrum

Book ID
109332584
Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
690 KB
Volume
14
Category
Article
ISSN
1574-6976

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Construction of an Escherichia coli K-12
Construction of an Escherichia coli K-12 strain deleted for manganese and iron superoxide dismutase genes and its use in cloning the iron superoxide dismutase gene of Legionella pneumophila
โœ Steinman, HowardM. ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› Springer ๐ŸŒ English โš– 547 KB

An Escherichia coli K-12 strain deleted for sodA and sodB (manganese and iron superoxide dismutases) was constructed and characterized by Southern blotting, enzyme assays, and physiological analyses. The sod deletion strain was used to clone the iron superoxide dismutase gene of Legionella pneumophi