✦ LIBER ✦

Construction of an Escherichia coli K-12 strain deleted for manganese and iron superoxide dismutase genes and its use in cloning the iron superoxide dismutase gene of Legionella pneumophila

✍ Scribed by Steinman, HowardM.

Publisher: Springer
Year: 1992
Tongue: English
Weight: 547 KB
Volume: 232
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00266247

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✦ Synopsis

An Escherichia coli K-12 strain deleted for sodA and sodB (manganese and iron superoxide dismutases) was constructed and characterized by Southern blotting, enzyme assays, and physiological analyses. The sod deletion strain was used to clone the iron superoxide dismutase gene of Legionella pneumophila by complementation to paraquat resistance.