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Induction of sister-chromatid exchange in human lymphocytes by vitamin B6

✍ Scribed by J. Dozi-Vassiliades; D. Mourelatos; A. Myrtsiotis


Book ID
119092604
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
187 KB
Volume
124
Category
Article
ISSN
0165-1218

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This study examined the effect of pyrimethamine (PYR) on sister chromatid exchanges (SCEs) in human lymphocytes. PYR is a folic acid antagonist used for the treatment of malaria and toxoplasmosis. PYR was added to peripheral blood lymphocyte cultures at three different doses: 0.05, 0.1, and 0.2 mg/m

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The number of sister chromatid exchanges (SCE) per metaphase was determined in normal human lymphocytes by the Hoechst-Giemsa procedure. UV C irradiation promotes SCE dose-dependent in the range of 0-40 mJ/cm2. No difference of this phenomenon may be observed, compairing young volunteers (20-40 year