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Sister chromatid exchange (SCE) in human lymphocytes

โœ Scribed by Flavia Zanzoni; J. W. A. Baumann; E. G. Jung

Publisher
Springer-Verlag
Year
1979
Tongue
English
Weight
226 KB
Volume
265
Category
Article
ISSN
0340-3696

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โœฆ Synopsis

The number of sister chromatid exchanges (SCE) per metaphase was determined in normal human lymphocytes by the Hoechst-Giemsa procedure. UV C irradiation promotes SCE dose-dependent in the range of 0-40 mJ/cm2. No difference of this phenomenon may be observed, compairing young volunteers (20-40 years) with a 60-80-year-old group.

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## Abstract The utility of the sister chromatid exchange (SCE) assay for human population studies is potentially limited by the variability associated with individual baseline SCE frequencies. This investigation identifies and quantifies the major sources of preparative and biological variation ass