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Induction of Apoptosis by a Dominant Negative H-RAS Mutant (116Y) in K562 Cells

✍ Scribed by Norio Sakai; Yoshifumi Ogiso; Hisakazu Fujita; Hidemichi Watari; Takao Koike; Noboru Kuzumaki

Book ID: 115601884
Publisher: Elsevier Science
Year: 1994
Tongue: English
Weight: 432 KB
Volume: 215
Category: Article
ISSN: 0014-4827
DOI: 10.1006/excr.1994.1324

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FIGURE 4 -Inhibition of the phosphorylation of Erk2 by N116Y. Cells infected with 200 MOI of AdCMV or AdCMV-N116Y were starved for 24 hr and then stimulated by EGF (20 ng/ml) for 10 min. Cell lysate (30 µg) were separated on 12% SDS-PAGE, electroblotted onto nitrocellulose membrane and probed with E

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