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The dominant negative H-ras mutant, N116Y, suppresses growth of metastatic human pancreatic cancer cells in the liver of nude mice

โœ Scribed by Takeuchi, M; Shichinohe, T; Senmaru, N; Miyamoto, M; Fujita, H; Takimoto, M; Kondo, S; Katoh, H; Kuzumaki, N


Book ID
110038624
Publisher
Nature Publishing Group
Year
2000
Tongue
English
Weight
241 KB
Volume
7
Category
Article
ISSN
0969-7128

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FIGURE 4 -Inhibition of the phosphorylation of Erk2 by N116Y. Cells infected with 200 MOI of AdCMV or AdCMV-N116Y were starved for 24 hr and then stimulated by EGF (20 ng/ml) for 10 min. Cell lysate (30 ยตg) were separated on 12% SDS-PAGE, electroblotted onto nitrocellulose membrane and probed with E