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Suppressive effects of dominant negative ras mutant N116Y on transformed phenotypes of human bladder cancer cells

โœ Scribed by Takafumi Watanabe; Nobuo Shinohara; Ataru Sazawa; Yoshiyasu Kobayashi; Yoshifumi Ogiso; Takashi Kimura; Mitsuyoshi Takiguchi; Jun Yasuda; Akira Hashimoto; Tomohiko Koyanagi; Noboru Kuzumaki


Book ID
117469713
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
276 KB
Volume
149
Category
Article
ISSN
0304-3835

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FIGURE 4 -Inhibition of the phosphorylation of Erk2 by N116Y. Cells infected with 200 MOI of AdCMV or AdCMV-N116Y were starved for 24 hr and then stimulated by EGF (20 ng/ml) for 10 min. Cell lysate (30 ยตg) were separated on 12% SDS-PAGE, electroblotted onto nitrocellulose membrane and probed with E