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Induction and disappearance of DNA strand breaks in human peripheral blood lymphocytes and fibroblasts treated with methyl methanesulfonate

✍ Scribed by Michael E.T.I. Boerrigter; Erik Mullaart; Jan Vijg

Book ID
115892579
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
685 KB
Volume
192
Category
Article
ISSN
0014-4827

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In vitro exposure of normal human diploid fibroblasts (strain VH-10) to ethylene oxide (EtO) induced DNA strand breaks in the dose range of 2.5-30 mMh of EtO. Alkaline DNA unwinding (ADU), neutral filter elution (NFE), pulsed field gel electrophoresis (PFGE), and the comet assay were used to measure