Baculovirus expression vectors are widely used for expressing heterologous proteins in cultured insect cells. Recent advances include further development of the system for production of multi-subunit protein complexes, co-expression of protein-modifying enzymes to improve heterologous protein produc
Inducible vectors for expression in mammalian cells
โ Scribed by Geoffrey T. Yarranton
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 594 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0958-1669
No coin nor oath required. For personal study only.
โฆ Synopsis
The most recent developments in mammalian cell inducible expression systems have involved the use of bacterial gene control elements and viral transactivator proteins. The combination of hybrid viral transactivator and bacterial repressor proteins, and simple chemical inducers can provide induction ratios of over 1000-fold. These developments will have applications in both cell-based research and the generation of transgenic animals.
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We constructed tricistronic expression vectors for the simultaneous and coordinated expression of three independent genes in mammalian cells. One single promoter allows high level and, in some vectors, adjustable transcription of all three cistrons. Whereas the first cistron is translated in a cap-d
Abbreviations AcMNPV Autographa califomica multiple nuclear polyhedrosis virus Ea Estigmene acrea ## GST glutathione S-transferase LIC ligation-independent cloning PCR polymerase chain reaction VLP virus-like particle
Of the wide variety of regulatory systems that have been developed to control gene expression in higher eukaryotes, those utilizing elements from prokaryotic systems presently achieve the highest specificity. In particular, systems based on the repressor/operator elements of an Escherichia coil tetr