The cDNA encoding neuroparsin A, a polytropic neurohormone of the locust, Locusta migratoria, was inserted into the genome of Autographa californica nuclear polyhedrosis virus such that transcription was under control of the p10 promoter. A polypeptide having the same charge and the same apparent mo
Baculovirus vectors for expression in insect cells
β Scribed by Ian Jones; Yuko Morikawa
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 424 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0958-1669
No coin nor oath required. For personal study only.
β¦ Synopsis
Abbreviations AcMNPV Autographa califomica multiple nuclear polyhedrosis virus Ea
Estigmene acrea
GST
glutathione S-transferase LIC ligation-independent cloning PCR polymerase chain reaction VLP virus-like particle
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## Abstract The baculovirus expression system is one of the most popular methods used for the production of recombinant proteins but has several complex steps which have proved inherently difficult to adapt to a multiβparallel process. We have developed a bacmid vector that does not require any for
Baculovirus expression vectors are widely used for expressing heterologous proteins in cultured insect cells. Recent advances include further development of the system for production of multi-subunit protein complexes, co-expression of protein-modifying enzymes to improve heterologous protein produc