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Expression of neuroparsin cDNA in insect cells using baculovirus vectors

✍ Scribed by J. Girardie; H. Chaabihi; B. Fournier; M. Lagueux; A. Girardie

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
164 KB
Volume
36
Category
Article
ISSN
0739-4462

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✦ Synopsis

The cDNA encoding neuroparsin A, a polytropic neurohormone of the locust, Locusta migratoria, was inserted into the genome of Autographa californica nuclear polyhedrosis virus such that transcription was under control of the p10 promoter. A polypeptide having the same charge and the same apparent molecular weight as the authentic neuroparsin A and that was reactive against neuroparsin immune serum was produced in recombinant virus-infected lepidopteran cell lines but not in control virus-infected cells. The baculovirusexpressed polypeptide was purified by two steps of liquid chromatography (anion exchange and reversed phase) which were previously used to purify the natural neuroparsin. The purified baculovirus-expressed polypeptide enhanced fluid reabsorption of everted rectum preparations, as did the natural neuroparsin. Thus, this gene expression system produced a polypeptide identical to authentic neuroparsin. Arch. Insect Biochem. Physiol. 36:11-23, 1997.

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