✦ LIBER ✦

Increased PKC activity in cultured human keratinocytes and fibroblasts after treatment with 1α,25-dihydroxyvitamin D3

✍ Scribed by Nancy M. Hanafin; Kelly Scott Persons; Michael F. Holick

Publisher: John Wiley and Sons
Year: 1995
Tongue: English
Weight: 751 KB
Volume: 57
Category: Article
ISSN: 0730-2312
DOI: 10.1002/jcb.240570220

No coin nor oath required. For personal study only.

✦ Synopsis

la,25-Dihydroxyvitarnin D3 (10-l2 M to M) caused a dose dependent increase in PKC activity in the solubilized membrane fractions of cultured human keratinocytes and in the cytosolic fractions of cultured human fibroblasts. Maximum activity was induced by 1 a,25-dihydroxyvitamin D3 at 24 h. Sphingosine, which is believed to inhibit PKC mediated biological responses, blunted 1 ~, 2 5 ( 0 H ) ~D ~' s inducement of PKC activity in both keratinocytes and fibroblasts. Identical hormone treatment of vitamin D receptor deficient fibroblasts did not increase PKC activity.

Treatment of keratinocytes and fibroblasts with 1 j3,25-dihydroxyvitamin D3 , which i s believed to be ineffective in inducinggenomic responses, did not induce PKC activity.

📜 SIMILAR VOLUMES

Metabolism of 3H-1α,25-dihydroxyvitamin

Metabolism of 3H-1α,25-dihydroxyvitamin D3 in cultured human keratinocytes

✍ Swapna Ray; Dr. Rahul Ray; Michael F. Holick 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 462 KB

In the present investigation we studied the metabolism of 1 cu,25-dihydroxy-[l P-3H]vitamin D3 (3H-1 ,25(OH),D3) in culture-grown human keratinocytes (CHK). O u r results showed that the cellular uptake of 3H-1,25(OH)2D3, upon incubation with CHK, occurred very rapidly; and it paralleled a decrease

Hexafluoro-1,25-dihydroxyvitamin D3 has

Hexafluoro-1,25-dihydroxyvitamin D3 has markedly increased potency in inhibiting proliferation of cultured human keratinocytes compared with 1,25-dihydroxyvitamin D3

✍ T.C. Chen; M.F. Holick 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 409 KB

## Background: Although topical 1,25-dihydroxyvitamin d3 (calcitriol, 1, 25(oh)2d3) and its analogues, calcipotriol and tacalcitol, are effective for patients with psoriasis, some patients show little or no response. there is a need to develop more potent analogues of 1, 25(oh)2d3. hexafluoro-1,25(

Growth medium-dependent ERK1/2 and AP-1

Growth medium-dependent ERK1/2 and AP-1 activity in cultured normal human keratinocytes modulates 1α,25-dihydroxyvitamin D3-induced differentiation

✍ Claus Johansen; Knud Kragballe; Lars Iversen 📂 Article 📅 2003 🏛 Springer-Verlag 🌐 English ⚖ 205 KB

1,25(OH)2D3 increases calcium and phosph

1,25(OH)2D3 increases calcium and phosphatidylinositol metabolism in differentiating cultured human keratinocytes

✍ Julia A. MacLaughlin; Lewis C. Cantley; Michael F. Holick 📂 Article 📅 1990 🏛 Elsevier Science 🌐 English ⚖ 693 KB

Treatment with 1,25-dihydroxyvitamin D3

Treatment with 1,25-dihydroxyvitamin D3 reduces impairment of human osteoblast functions during cellular aging in culture

✍ Marie Kveiborg; Suresh I.S. Rattan; Brian F.C. Clark; Erik F. Eriksen; Moustapha 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 227 KB 👁 1 views

Differentiating and antitumor activities

Differentiating and antitumor activities of 1α,25-dihydroxyvitamin D3 in vitro and 1α-hydroxyvitamin D3 in vivo on human osteosarcoma

✍ Dr. Hiroyuki Tsuchiya; Hajime Morishita; Katsuro Tomita; Yoshimichi Ueda; Motohi 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 813 KB

## Abstract The differentiating and antitumor activities of 1α,25‐dihydroxyvitamin D~3~ (1α,25(OH)~2~D~3~) in vitro and 1α‐hydroxyvitamin D~3~ (1α(OH)D~3~) in vivo were studied with a human osteosarcoma cell line (OST strain). Antitumor activity was estimated with the use of 3‐(4,5‐dimethylthiazol)