In the present investigation we studied the metabolism of 1 cu,25-dihydroxy-[l P-3H]vitamin D3 (3H-1 ,25(OH),D3) in culture-grown human keratinocytes (CHK). O u r results showed that the cellular uptake of 3H-1,25(OH)2D3, upon incubation with CHK, occurred very rapidly; and it paralleled a decrease
1,25(OH)2D3 increases calcium and phosphatidylinositol metabolism in differentiating cultured human keratinocytes
β Scribed by Julia A. MacLaughlin; Lewis C. Cantley; Michael F. Holick
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 693 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0955-2863
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la,25-Dihydroxyvitarnin D3 (10-l2 M to M) caused a dose dependent increase in PKC activity in the solubilized membrane fractions of cultured human keratinocytes and in the cytosolic fractions of cultured human fibroblasts. Maximum activity was induced by 1 a,25-dihydroxyvitamin D3 at 24 h. Sphingosi
## Background: Although topical 1,25-dihydroxyvitamin d3 (calcitriol, 1, 25(oh)2d3) and its analogues, calcipotriol and tacalcitol, are effective for patients with psoriasis, some patients show little or no response. there is a need to develop more potent analogues of 1, 25(oh)2d3. hexafluoro-1,25(