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Metabolism of 3H-1α,25-dihydroxyvitamin D3 in cultured human keratinocytes

✍ Scribed by Swapna Ray; Dr. Rahul Ray; Michael F. Holick

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
462 KB
Volume
59
Category
Article
ISSN
0730-2312

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✦ Synopsis

In the present investigation we studied the metabolism of 1 cu,25-dihydroxy-[l P-3H]vitamin D3 (3H-1 ,25(OH),D3) in culture-grown human keratinocytes (CHK). O u r results showed that the cellular uptake of 3H-1,25(OH)2D3, upon incubation with CHK, occurred very rapidly; and it paralleled a decrease in the concentration of 3H-I ,25(OH),D3 in the medium. The amount of 3H-calcitroic acid, on the other hand, increased slowly in the medium, while the concentration of 3H-calcitroic acid in the cell remained undetectable during the whole period of incubation. When the cells were preincubated with 1,25(OH)*D3 (10-8M), conversion of 3H-1,25(OH)2D3 to 3H-calcitroic acid increased almost twofold, indicating that 1 ,25(OH),D3 catalyzed its own catabolism.

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