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Incorporation of H3-uridine and the isolation and characterization of RNA from squid axon

✍ Scribed by S. Fischer; P. Gariglio; E. Tarifeño

Publisher
John Wiley and Sons
Year
1969
Tongue
English
Weight
594 KB
Volume
74
Category
Article
ISSN
0021-9541

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✦ Synopsis

Ha-Uridine microinjected in the giant axons of the squid is incorporated in a TCA insoluble material. There is no difference between stimulated and resting axons as to the amount incorporated.

The amount incorporated is increased if the stimulation precedes the microinjection of the tracer.

RNA was purified and characterized from the axoplasm, axon sheaths and from a purified membrane preparation obtained from squid retinal nerve.

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