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The presence of transfer RNA in the axoplasm of the squid giant axon

✍ Scribed by Black, M. M. ;Lasek, R. J.

Publisher: John Wiley and Sons
Year: 1977
Tongue: English
Weight: 602 KB
Volume: 8
Category: Article
ISSN: 0022-3034
DOI: 10.1002/neu.480080306

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✦ Synopsis

Abstract

Previous work has revealed that 4S RNA is the primary species of RNA in the axoplasm from the giant axons of the squid and Myxicola. This study shows that axoplasmic 4S RNA from the squid giant axon has the functional properties of tRNA. Axoplasmic RNA was charged with amino acids by aminoacyl‐tRNA synthetases prepared from squid brain. The aminoacylation was prevented by incubating the RNA with RNase prior to running the reaction. The amino acid‐RNA complex was labile at pH 9, which is characteristic of the acyl linkage between an amino acid and its tRNA. Aminoacyl‐tRNA synthetase activity was also present in the axoplasm, primarily in the soluble fraction.

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