Isolation and characterization of the nuclear matrix from the male xenopus laevis following estrogen administration: Kinetics of [3H] uridine incorporation

✍ Scribed by Snead, Henry W. ;McDonald, Thomas F. ;Baker, Mary D. ;Lanclos, Kenneth D.

• Publisher: Wiley (John Wiley & Sons)
• Year: 1979
• Tongue: English
• Weight: 616 KB
• Volume: 12
• Category: Article
• ISSN: 0091-7419
• DOI: 10.1002/jss.400120407

✦ Synopsis

Abstract

At various times following estorgen administration, the nuclear matrix was isolated from the liver of male Xenopus laevis by sucrose gradient centrifugation of nuclei treated with a high‐salt buffer and DNase I in the presence of a proteolytic inhibitor (PMSC ‐ phenylmethyl sulfonyl chloride). Electron micrographs of the nuclear matrix demonstrate a sponge‐like network attached to a well‐defined inner envelope with a ribosome‐free outer envelope. Chemical analyses show that the HSB‐DNase‐treated nuclei consist of 16% DNA, 2% RNA, and 82% protein, a composition that is consistent with that of nuclear matrices isolated from other species. The specific activity of the matrix‐associated RNA following estrogen treatment appears to be maximally enhanced after 5 h and decreases until approximately 12 h, when the activity begins to increase again.