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Inactivation of Horseradish Peroxidase by Phenoxyl Radical Attack

✍ Scribed by Huang, Qing; Huang, Qingguo; Pinto, Roger A.; Griebenow, Kai; Schweitzer-Stenner, Reinhard; Weber, Walter J.


Book ID
121504906
Publisher
American Chemical Society
Year
2005
Tongue
English
Weight
152 KB
Volume
127
Category
Article
ISSN
0002-7863

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Horseradish peroxidase which had been aminated by periodate oxidation and reductive amination was purified by cation-exchange chromatography on S-Sepharose. Instead of the expected single peak of aminated enzyme, two distinct peaks of protein were eluted from the column. Evaluation of the protein in