In vivo oxygen tension and temperature: Simultaneous determination using 19F NMR spectroscopy of perfluorocarbon

✍ Scribed by Dr. Ralph P. Mason; Himu Shukla; Peter P. Antich

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 590 KB
• Volume: 29
• Category: Article
• ISSN: 0740-3194
• DOI: 10.1002/mrm.1910290304

✦ Synopsis

Abstract

A novel technique is presented to measure in vivo simulataneously oxygen tension and temperature using ^19^F NMR spectroscopy of perfluorocarbon. This work examines the variation with oxygen tension (pO~2~) and temperature of the individual spin lattice relaxation rates (R~1~) of the ^19^F resonances of the perfluorocarbon emulsion Oxypherol‐ET. A linear relationship between R~1~ and pO~2~ is confirmed for all the resonances at any specific temperature in the range 27–50°C. Similarly, a linear relationship is determined between R~1~ and temperature at any specific pO~2~ in this temperature range. Each resonance behaves uniquely with respect to temperature and pO~2~ and consideration of 2 or more resonances uniquely defines pO~2~ and temperature simultaneously, and unambiguously. This concept is demonstrated in vivo in a murine tumor and perfused rat heart, where pO~2~ and temperature were both determined without prior knowledge.