Tissue oxygenation: A novel determination using 19F surface coil NMR spectroscopy of sequestered perfluorocarbon emulsion

✍ Scribed by Ralph P. Mason; Ray L. Nunnally; Peter P. Antich

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 537 KB
• Volume: 18
• Category: Article
• ISSN: 0740-3194
• DOI: 10.1002/mrm.1910180109

✦ Synopsis

Abstract

This work examines the variation with oxygen tension (__p__O~2~) of the individual spin‐lattice relaxation times (T~I~) of the ^19^F resonances of the perfluorocarbon emulsion Oxy‐pherol‐ET (FC‐43). A linear relationship between 1/T~1~ and __p__O~2~ has been confirmed for all four resonances at any specific temperature. Using a saturation recovery sequence, T~1~ has been successfully measured using surface coil NMR spectroscopy. This has facilitated measurement of T~I~ in vivo in a subcutaneous murine tumor. Mice were predosed with Oxypherol‐ET emulsion; following complete vascular clearance of the pertluorocarbon, ^19^F signal was observed specifically from material sequestered in tissue, thus avoiding flow artifacts. Comparison of the __p__O~2~ estimated from each of the ^19^F resonances provided an internal consistency check. A __p__O~2~ = 0.1 ± 2.2% was determined in a Meth‐A murine tumor. When the mouse breathed carbogen (95% O~2~, 5% CO~2~) no significant change in tumor __p__O~2~ was detected, whereas the __p__O~2~ in the liver showed a distinct increase. © 1991 Academic Press. Inc.