𝔖 Bobbio Scriptorium
✦   LIBER   ✦

In Vivo Gene Transfer by Low-Volume Jet Injection

✍ Scribed by Régis Cartier; Shuxun V. Ren; Wolfgang Walther; Ulrike Stein; Albert Lewis; Peter M. Schlag; Minglin Li; Priscilla A. Furth

Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
78 KB
Volume
282
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

around 7% of chimeric clones assuming that an average full-length ds cDNA contains six RsaI sites (1 of 14; total of 14 ends in seven cDNA fragments). Unlike the 5Ј end cDNA fragment, the 3Ј end fragment has two RsaI blunt ends because the cDNA synthesis primer used in SSH contains a RsaI site.

In conclusion, chimeric cDNA clones containing transcripts from different genes are formed during SSH and the subsequent PCR. These chimeric clones usually contain a regenerated RsaI site and can be identified easily by RsaI digestion following PCR amplification of the chimeric cDNA sequences.

Acknowledgment. We thank Dr. Billie M. Moats-Staats for critical reading of the manuscript and helpful discussions.

📜 SIMILAR VOLUMES

Needleless in vivo gene transfer into mu
Needleless in vivo gene transfer into muscles by jet injection in combination with electroporation
✍ Mayumi Horiki; Eiji Yamato; Hiroshi Ikegami; Toshio Ogihara; Jun-ichi Miyazaki 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 181 KB

## Abstract Previously, we have established an __in vivo__ electroporation method for gene transfer into muscle by injection of DNA with a needle followed by electric pulse delivery using needle‐type electrodes and proved that this method is effective for the systemic delivery of cytokines. To perf

Use of the nuclease inhibitor aurintrica
Use of the nuclease inhibitor aurintricarboxylic acid (ATA) for improved non-viral intratumoral in vivo gene transfer by jet-injection
✍ Wolfgang Walther; Ulrike Stein; Robert Siegel; Iduna Fichtner; Peter M. Schlag 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 230 KB

## Abstract ## Background Stability, integrity and retention of the DNA within the targeted tissue is decisive for efficient gene transfer using naked DNA. Pre‐clinical and clinical studies require reproducible transfection rates by preventing rapid degradation of naked DNA in the transduced tissu

Electroporation-mediated ex vivo gene tr
Electroporation-mediated ex vivo gene transfer into graft not requiring injection pressure in orthotopic liver transplantation
✍ Shogo Kobayashi; Keizo Dono; Shiro Takahara; Yoshitaka Isaka; Enyu Imai; Lu Zhen 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 187 KB

## Abstract ## Background We investigated optimum conditions for __ex vivo__ gene transfer into liver grafts by plasmid injection via the portal vein combined with electroporation in rat liver transplantation. ## Methods Anesthetized 9‐week‐old male Shionogi‐Wistar rats were used as donors and r

Gene transfer in swine embryos by inject
Gene transfer in swine embryos by injection of cells infected with retrovirus vectors
✍ Petters, Robert M. ;Shuman, Ruth M. ;Johnson, Bryan H. ;Mettus, Richard V. 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 441 KB

Key components for gene transfer to swine embryos using an avian retrovirus are described. A replication-defective reticuloendotheliosis (REV) viral vector can infect and be expressed in pig embryo fibroblasts (PEF). Infection with a replication-competent vector (REV-A) indicates a presumptive block

Maximizing the in vivo efficiency of gen
Maximizing the in vivo efficiency of gene transfer by means of nonviral polymeric gene delivery vehicles
✍ Ales Prokop; Evgenii Kozlov; William Moore; Jeffrey M. Davidson 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 152 KB

We have screened many synthetic and natural polymers for their ability to facilitate gene delivery in vivo into subcutaneous tissue. We postulated that gene delivery polymeric vehicles could control the chemical and biological stability of plasmid as well as their colloidal and surface properties, a