𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Maximizing the in vivo efficiency of gene transfer by means of nonviral polymeric gene delivery vehicles

✍ Scribed by Ales Prokop; Evgenii Kozlov; William Moore; Jeffrey M. Davidson

Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
152 KB
Volume
91
Category
Article
ISSN
0022-3549

No coin nor oath required. For personal study only.

✦ Synopsis

We have screened many synthetic and natural polymers for their ability to facilitate gene delivery in vivo into subcutaneous tissue. We postulated that gene delivery polymeric vehicles could control the chemical and biological stability of plasmid as well as their colloidal and surface properties, and this may lead to enhanced gene delivery in vivo. The screening was based on reporter gene (luciferase) expression using a plasmid containing the cytomegalovirus promoter. The control of charge density of resulting particles was achieved by selecting different ratios of polymer to plasmid mass as well as polymer chemistry. As opposed to in vitro observations, only polymers that lead to negatively charged particles exhibited an in vivo activity, in opposite to in vitro case. Such polymers exhibited relatively low positive charge chemistry (or neutral charge). The most potent polymeric vehicles were members of a Tetronic polymer series (synthetic polyethylene±polypropylene block copolymers; BASF, Mount Olive, NJ) or from a noncharged polyvinylpyrrolidine formulation. Plasmid dose response and time relationships were established for some polymers. Results were applied to the redesign of particles for biolistic (gene gun) plasmid gene delivery.

📜 SIMILAR VOLUMES

Analysis of the intracellular barriers e
Analysis of the intracellular barriers encountered by nonviral gene carriers in a model of spatially controlled delivery to neurons
✍ Jamie M. Bergen; Suzie H. Pun 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 241 KB

## Abstract ## Background Neuron‐specific, nonviral gene delivery vehicles are useful tools for the potential treatment of neurological disease and spinal cord injury. For minimally invasive, peripheral administration, gene carriers must efficiently mediate uptake at axon terminals, retrograde axo

Efficient gene delivery into murine ovar
Efficient gene delivery into murine ovarian cells by intraovarian injection of plasmid DNA and subsequent in vivo electroporation
✍ Masahiro Sato; Maya Tanigawa; Natsuko Kikuchi; Shingo Nakamura; Minoru Kimura 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 396 KB

## Abstract Summary: We describe the use of direct injection of circular plasmid DNA and subsequent in vivo electroporation (EP) for efficient gene delivery to the ovarian cells, including follicular cells and oocytes of mice. When Trypan blue (TB) was injected into the central portion of an ovary

Successful gene therapy of the Gunn rat
Successful gene therapy of the Gunn rat by in vivo neonatal hepatic gene transfer using murine oncoretroviral vectors
✍ Marta Bellodi-Privato; Dominique Aubert; Virginie Pichard; Anne Myara; François 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 250 KB

Crigler-Najjar type 1 disease (CN1) is a rare inherited metabolic disease characterized by complete absence of hepatic UDP-glucuronosyl transferase (UGT1), resulting in high levels of unconjugated bilirubin. CN1 is an attractive candidate disease for gene therapy. Here we show that in vivo neonatal

Targeting of the glucocorticoid hormone
Targeting of the glucocorticoid hormone receptor with plasmid DNA comprising glucocorticoid response elements improves nonviral gene transfer efficiency in the lungs of mice
✍ Petra Dames; Andreas Laner; Christof Maucksch; Manish Kumar Aneja; Carsten Rudol 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 894 KB

## Abstract ## Background It has been previously demonstrated that plasmid DNA transport into the nucleus could be increased by transcription factor binding. We chose the glucocorticoid responsive element (GRE) which binds to the glucocorticoid receptor (GR), a transcription factor which is shuttl

Relationship between the physical shape
Relationship between the physical shape and the efficiency of oligomeric chitosan as a gene delivery system in vitro and in vivo
✍ M. Köping-Höggård; Y. S. Mel'nikova; K. M. Vårum; B. Lindman; P. Artursson 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 351 KB

## Abstract ## Background Chitosans of high molecular weights have emerged as efficient nonviral gene delivery systems, but the properties and efficiency of well‐defined low molecular weight chitosans (<5 kDa) have not been studied. We therefore characterized DNA complexes of such low molecular we

In vivo targeted gene transfer in skin b
In vivo targeted gene transfer in skin by the use of laser-induced stress waves
✍ Makoto Ogura; Shunichi Sato; Kuniaki Nakanishi; Maki Uenoyama; Tetsuro Kiyozumi; 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 135 KB

## Abstract ## Background and Objectives Much interest has been shown in the use of lasers for nonviral targeted gene transfer, since the spatial characteristics of laser light are quite well defined. The aim of this study was to demonstrate in vivo gene transfer by the use of laser‐induced stress