✦ LIBER ✦

Gene transfer in swine embryos by injection of cells infected with retrovirus vectors

✍ Scribed by Petters, Robert M. ;Shuman, Ruth M. ;Johnson, Bryan H. ;Mettus, Richard V.

Publisher: John Wiley and Sons
Year: 1987
Tongue: English
Weight: 441 KB
Volume: 242
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1402420111

No coin nor oath required. For personal study only.

✦ Synopsis

Key components for gene transfer to swine embryos using an avian retrovirus are described. A replication-defective reticuloendotheliosis (REV) viral vector can infect and be expressed in pig embryo fibroblasts (PEF). Infection with a replication-competent vector (REV-A) indicates a presumptive block to viral replication in PEF. Swine embryos obtained at the morula stage can be cultured in vitro to the blastocyst stage, injected with retrovirus helper cells or quail embryo fibroblasts producing REV, and transferred to recipient swine with survival to at least 6 wk of gestation.

📜 SIMILAR VOLUMES

Retinal cell type expression specificity

Retinal cell type expression specificity of HIV-1-derived gene transfer vectors upon subretinal injection in the adult rat: influence of pseudotyping and promoter

✍ Alexis-Pierre Bemelmans; Sébastien Bonnel; Leïla Houhou; Noëlle Dufour; Emeline 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 321 KB

Background Gene therapy, and particularly gene restoration, is currently a great hope for non-curable hereditary retinal degeneration. Clinical applications require a gene transfer vector capable of accurately targeting particular cell types in the retina. To develop such a vector, we compared the e

Generation of stable, high-producing cho

Generation of stable, high-producing cho cell lines by lentiviral vector-mediated gene transfer in serum-free suspension culture

✍ Agata Oberbek; Mattia Matasci; David L. Hacker; Florian M. Wurm 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 640 KB

Suppression of the tumorigenic growth of

Suppression of the tumorigenic growth of Burkitt's lymphoma cells in immunodeficient mice by cytokine gene transfer using EBV-derived episomal expression vectors

✍ Susanne Mücke; Andreas Draube; Axel Polack; Michael Pawlita; Nadia Massoudi; And 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 French ⚖ 124 KB 👁 2 views

Epstein-Barr virus (EBV)-based expression vectors were tested for cytokine gene transfer-mediated induction of an immune response against human lymphoma cells. These vectors express the EBV latent gene EBNA 1 and carry the EBV latent origin of replication (ori P) for episomal replication in transfec

Acquired immunity in nude mice induced b

Acquired immunity in nude mice induced by expression of the IL-2 or IL-4 gene in human pancreatic carcinoma cells and anti-tumor effect generated by In vivo gene transfer using retrovirus

✍ Masaki Kimura; Yu Yoshida; Mitsuro Narita; Keizo Takenaga; Toshinao Takenouchi; 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 French ⚖ 330 KB 👁 2 views

We have examined the anti-tumor effect in nude mice caused by human pancreatic cancer cells (AsPC-1) modified to secrete IL-2 or IL-4. Loss of tumorigenicity of cytokineproducing, but not wild-type, cells was observed despite their unaltered in vitro proliferation rates; and these anti-tumor effects

Gene transfer of human TCR in primary mu

Gene transfer of human TCR in primary murine T cells is improved by pseudo-typing with amphotropic and ecotropic envelopes

✍ Nadine M. C. Pouw; Elike J. Westerlaken; Ralph A. Willemsen; Reno Debets 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 333 KB

## Abstract ## Background T cell receptor (TCR) gene therapy represents an attractive anti‐cancer treatment but requires further optimization of its efficacy and safety in clinically relevant models, such as those using a tumor antigen and TCR of human origin. Currently, however, there is no conse

Inhibition of initiator-induced SV40 gen

Inhibition of initiator-induced SV40 gene amplification in SV40-transformed Chinese hamster cells by infection with a defective parvovirus

✍ Jörg R. Schlehofer; Regine Heilbronn; Brigitte Georg-Fries; Harald Zur Hausen 📂 Article 📅 1983 🏛 John Wiley and Sons 🌐 French ⚖ 469 KB 👁 1 views

## Abstract Amplification of SV40 genes in SV40‐transformed Chinese hamster embryo cells (CO631) by chemical carcinogens as well as by herpes simplex virus infection can be inhibited by infection with the defective parvovirus, AAV‐5. This is shown by __in situ__ hybridization with SV40 DNA of AAV‐5