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In vivo flow 31P FT-NMR spectroscopy of a nematode parasite

✍ Scribed by S. N. Thompson; E. G. Platzer; R. W.-K. Lee

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
385 KB
Volume
16
Category
Article
ISSN
0740-3194

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✦ Synopsis

Abstract

^31^P NMR spectroscopy of a parasitic nematode, Steinernema carpocapsae, is described. In vivo spectra were generated from nematode suspensions continuously circulating through the spectrometer. By flowing the organism, saturation effects were avoided and short in‐terpulse delays significantly reduced spectral generation time. To maximize sensitivity, 90Β° pulses were employed. Changes in energetic status in response to oxygen availability were easily and rapidly monitored in circulating nematodes. Β© 1990 Academic Press, Inc.

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