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Phosphoarginine-Adenosine Triphosphate Exchange Detected in Vivo in a Microscopic Nematode Parasite by Flow 31P FT-NMR Spectroscopy

✍ Scribed by S. N. Thompson; E. G. Platzer; R.W.-K. Lee

Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 404 KB
Volume: 28
Category: Article
ISSN: 0740-3194
DOI: 10.1002/mrm.1910280213

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✦ Synopsis

Abstract

In vivo flow ^31^P NMR spectroscopy of a microscopic nematode, Steinernema carpocapsae, is described. Long‐term viability was maintained during analysis by continuous circulation of an oxygenated suspension of the parasite through an NMR spectrometer. Saturation transfer and inversion recovery were employed under flowing conditions to investigate the kinetics of phosphoarginine ⇌ adenosine triphosphate exchange. The kinetic constants for the forward and reverse reactions were 0.37/s and 1.45/s, respectively. This report is the first to demonstrate a functional phosphagen kinase in the metabolism of a parasitic helminth.© 1992 Academic Press,Inc.

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In vivo flow 31P FT-NMR spectroscopy of a nematode parasite

✍ S. N. Thompson; E. G. Platzer; R. W.-K. Lee 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 385 KB 👁 1 views

## Abstract ^31^P NMR spectroscopy of a parasitic nematode, __Steinernema carpocapsae__, is described. __In vivo__ spectra were generated from nematode suspensions continuously circulating through the spectrometer. By flowing the organism, saturation effects were avoided and short in‐terpulse delay