Part I: Introduction1. The Past, Present and Future of Chemical Risk Assessment Alice LimoncielPart II: Systemic Organ Toxicity2. In Vitro Systems for Hepatotoxicity Testing Jan G. Hengstler, Seddik Hammad, Ahmed Ghallab, Raymond Reif and Patricio Godoy3. In Vitro Methods for Cardiotoxicity Testing Vaibhav Shinde, Umesh Chaudhari, Isaia Sotiriadou, Jurgen Hescheler and Agapios Sachinidis4. Renal Cell Culture Paul Jennings, Lydia Aschauer, Anja Wilmes and Gerhard Gstraunthaler5. Cellular Models for In Vitro Lung Toxicology Oliver H. WittekindtPart III: Neurotoxicity6. In Vitro Developmental Neurotoxicity Testing: Relevant Models and Endpoints Anna Bal-Price and Helena T. Hogberg7. Blood Brain Barrier In Vitro Models and Their Applications in Toxicology Anaelle Fabulas-Da Costa, Christophe Landry, Lucie Dehouck, Emmanuel Sevin, Marie-Pierre Dehouck, Romeo Cecchelli and Maxime CulotPart IV: Sensory Organ Models8. Ocular Toxicity Chantra Eskes, Erwin van Vliet, Michael Schaffer and Valerie Zuang9. In Vitro Models for Ototoxic Research Jochen Schacht and Michael G. LeitnerPart V: Other Organs and Aspects10. Dermal Toxicity: Skin Sensitization David Basketter and Silvia Casati11. Immunotoxicity Thomas Hartung12. Reproductive Toxicity Giovanna Lazzari and Cesare Galli13. Evaluating Endocrine Disruption In Vitro Patrick J. Devine14. Current and Emerging In Vitro Methods for Genotoxicity and Carcinogenicity Federica Madia, Pascal Phrakonkham, and Raffaella CorviPart VI: The Use of Stem Cells15. Use of Induced Pluripotent Stem Cells in Drug Toxicity Screening Caroline Rauch, Paul Jennings, and Anja Wilmes16. Human Stem/Progenitor Cell-Based Assays for Neurodevelopmental Toxicity Testing Ellen FritschePart VII: Techniques and Markers17. High Content Imaging Approaches for In Vitro Toxicology Marianne Uteng, Davide Germano, Kamal Kumar Balavenkatraman, Francois Pognan, and Armin Wolf18. Application of Omics Technologies to In Vitro Toxicology Nina V. Balmer, Tyna Dao, Marcel Leist, Kinga Vojnits, Tanja Waldmann, Liang Zhao, Helena T. Hogberg, and Erwin van Vliet19. Stress Response Pathways Martin O. Leonard, Alice Limonciel, and Paul Jennings20. Translational Biomarkers, In Vitro and In Vivo Anja Wilmes, Alice Limonciel, Martin O. Leonard, and Paul JenningsPart VIII: Challenges and Considerations21. Nanotoxicology Agnieszka Kinsner-Ovaskainen, Pascal Colpo, Jessica Ponti and Francois Rossi22. Consideration of Metabolism in In Vitro Cellular Systems Olavi Pelkonen, Jorma T. Ahokas, Jukka Hakkola, and Miia Turpeinen23. In Vitro Approaches to Predictive Biokinetics Bas J. Blaauboer24. Extrapolating In Vitro Results to Predict Human Toxicity Nadia Quignot, Jeremy Hamon, and Frederic Y. Bois25. Considerations in the Development of In Vitro Toxicity Testing Methods Intended for Regulatory Use Sandra Coecke, Gerard Bowe, Anne Milcamps, Camilla Bernasconi, Ann-Charlotte Bostroem, Gilles Bories, Salvador Fortaner, Jean-Michel Gineste, Varvara Gouliarmou, Ingrid Langezaal, Roman Liska, Emilio Mendoza, Siegfried Morath, Vittorio Reina, Iwona Wilk-Zasadna and Maurice Whelan.